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Anti-SOD2 Antibody (8Z611)
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Catalog No. TMAY-02405
Alias superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB
Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in WB,ELISA,IP.
Anti-SOD2 Antibody (8Z611)
Anti-SOD2 Antibody (8Z611)
😃Good
Catalog No. TMAY-02405Alias superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB
Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Human |
| Alias | superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB |
| Ig Type | Rabbit IgG |
| Clone | 8Z611 |
| Reactivity | Human |
| Verified Activity | 1. SOD2 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-SOD2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-SOD2 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 16 kDa. -Observed band size: 18 kDa. 2. Anti-SOD2 rabbit monoclonal antibody at 1:10000 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:25 kDa. -Observed band size:25 kDa. 3. Anti-SOD2 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: SOD2 konckout Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:24 kDa. -Observed band size:24 kDa(Validation Experiment) |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:2000-1:10000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human SOD2 (rh SOD2; TMPY-02044; P04179-1; Lys 25-Lys 222). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Superoxide dismutases (SOD) are important anti-oxidant enzymes that guard against superoxide toxicity. In humans, as in all mammals and most chordates, three forms of superoxide dismutase (SOD) are present: SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. Mitochondrial superoxide dismutase [SOD; manganese SOD (MnSOD) or SOD2] neutralizes highly reactive superoxide radical (O•-2), the first member in the plethora of mitochondrial reactive oxygen species. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human SOD2 protein (TMPY-02044) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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