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Anti-SOD2 Antibody (8Z611)

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Catalog No. TMAY-02405
Alias superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB

Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in WB,ELISA,IP.

Anti-SOD2 Antibody (8Z611)

Anti-SOD2 Antibody (8Z611)

😃Good
Catalog No. TMAY-02405Alias superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB
Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in WB,ELISA,IP.
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100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in WB,ELISA,IP.
Aliassuperoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB
Ig Type
Monoclonal Rabbit IgG
Clone
8Z611
Reactivity
Human
Verified Activity
1. SOD2 was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg HepG2 Whole Cell Lysate.

-2 µL anti-SOD2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-SOD2 rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 16 kDa.

-Observed band size: 18 kDa.

2. Anti-SOD2 rabbit monoclonal antibody at 1:10000 dilution.

-Lane A: Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:25 kDa.

-Observed band size:25 kDa.

3. Anti-SOD2 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: SOD2 konckout Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:24 kDa.

-Observed band size:24 kDa(Validation Experiment)
Application
WB,ELISA,IP
Recommended Dose
WB: 1:2000-1:10000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human SOD2 (rh SOD2; TMPY-02044; P04179-1; Lys 25-Lys 222).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundSuperoxide dismutases (SOD) are important anti-oxidant enzymes that guard against superoxide toxicity. In humans, as in all mammals and most chordates, three forms of superoxide dismutase (SOD) are present: SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. Mitochondrial superoxide dismutase [SOD; manganese SOD (MnSOD) or SOD2] neutralizes highly reactive superoxide radical (O•-2), the first member in the plethora of mitochondrial reactive oxygen species.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human SOD2 protein (TMPY-02044)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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