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Anti-SOD2 Antibody (8Z611) (Synonyms: superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB)

Catalog No. TMAY-02405 Copy Product Info
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Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in ELISA,IP,WB.

Anti-SOD2 Antibody (8Z611)

Copy Product Info
🥰Excellent
Catalog No. TMAY-02405
Synonyms superoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB

Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in ELISA,IP,WB.

Anti-SOD2 Antibody
(8Z611)
Pack SizePriceUSA StockGlobal StockQuantity
100 μL$2707-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-SOD2 Antibody (8Z611) is a Rabbit antibody targeting SOD2. Anti-SOD2 Antibody (8Z611) can be used in ELISA,IP,WB.
Synonymssuperoxide dismutase 2, mitochondrial, MVCD6, MNSOD, IPOB
Ig Type
Rabbit IgG
Clone
8Z611
Reactivity
Human
Specificity
Human SOD2/Mn-SOD
Verified Activity
1. SOD2 was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-SOD2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-SOD2 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 16 kDa.
-Observed band size: 18 kDa.
2. Anti-SOD2 rabbit monoclonal antibody at 1:10000 dilution.
-Lane A: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:25 kDa.
-Observed band size:25 kDa.
3. Anti-SOD2 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell Lysate.
-Lane B: SOD2 konckout Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:24 kDa.
-Observed band size:24 kDa(Validation Experiment)
Application
Recommended Dose
WB: 1:2000-1:10000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human SOD2 (rh SOD2; TMPY-02044; P04179-1; Lys 25-Lys 222).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundSuperoxide dismutases (SOD) are important anti-oxidant enzymes that guard against superoxide toxicity. In humans, as in all mammals and most chordates, three forms of superoxide dismutase (SOD) are present: SOD1 is located in the cytoplasm, SOD2 in the mitochondria, and SOD3 is extracellular. Mitochondrial superoxide dismutase [SOD; manganese SOD (MnSOD) or SOD2] neutralizes highly reactive superoxide radical (O•-2), the first member in the plethora of mitochondrial reactive oxygen species.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human SOD2 protein (TMPY-02044)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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