Glypican 3/GPC3 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00502

Glypican 3/GPC3 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 62 kDa and the accession number is Q3TWB2.

Glypican 3/GPC3 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-00502

Glypican 3/GPC3 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 62 kDa and the accession number is Q3TWB2.

Pack Size	Price	Availability
100 μg	$276	In Stock
200 μg	$489	7-10 days
500 μg	$1,040	7-10 days

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Product Information

Biological Activity	Measured by its ability to bind biotinylated human FGF basic in a functional ELISA.
Description	Glypican 3/GPC3 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 62 kDa and the accession number is Q3TWB2.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	Q3TWB2
Synonyms	OCI-5,glypican 3
Construction	A DNA sequence encoding the mouse GPC3 (NP_057906.2) (Met1-Pro558) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gln 25
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	62 kDa (predicted); 62 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in the lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue-dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceed both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy