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CD74 Protein, Human, Recombinant (His)

Catalog No. TMPY-01199
Synonyms: II, DHLAG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-γ, HLADG

CD74 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.4 kDa and the accession number is P04233-2.

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CD74 Protein, Human, Recombinant (His)
Pack Size Availability Price/USD Quantity
100 μg In stock $ 373.00
200 μg 5 days $ 659.00
500 μg 5 days $ 2,170.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2-400 ng/ml.
Description CD74 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.4 kDa and the accession number is P04233-2.
Species Human
Expression System HEK293 Cells
Tag N-His
Accession Number P04233-2
Synonyms II, DHLAG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-γ, HLADG
Construction The extracellular domain (Gln 73-Met 232) of Human CD74 isoform b (NP_004346.1) was fused with a polyhistidine tag at the N-terminus.
Protein Purity > 80 % as determined by SDS-PAGE

Molecular Weight 20.4 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.

References and Literature

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Keywords

CD74 Protein, Human, Recombinant (His) II DHLAG Ia-GAMMA CD74 molecule, major histocompatibility complex, class II invariant chain Ia-γ HLADG recombinant recombinant-proteins proteins protein

 

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