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Anti-FH/Fumarate Hydratase Antibody (5L839)
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Catalog No. TMAY-02240
Anti-FH/Fumarate Hydratase Antibody (5L839) is a Mouse antibody targeting FH/Fumarate Hydratase. Anti-FH/Fumarate Hydratase Antibody (5L839) can be used in WB,ELISA.
Anti-FH/Fumarate Hydratase Antibody (5L839)
Anti-FH/Fumarate Hydratase Antibody (5L839)
😃Good
Catalog No. TMAY-02240
Anti-FH/Fumarate Hydratase Antibody (5L839) is a Mouse antibody targeting FH/Fumarate Hydratase. Anti-FH/Fumarate Hydratase Antibody (5L839) can be used in WB,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-FH/Fumarate Hydratase Antibody (5L839) is a Mouse antibody targeting FH/Fumarate Hydratase. Anti-FH/Fumarate Hydratase Antibody (5L839) can be used in WB,ELISA. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 5L839 |
| Reactivity | Human |
| Verified Activity | Anti-FH mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:55 kDa. -Observed band size:47 kDa |
| Application | WB,ELISA |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fumarate Hydratase / FH (rh Fumarate Hydratase / FH; TMPY-02003; P07954-1; Ala 45-Lys 510). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Fumarate Hydratase (FH) is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. Fumarate Hydratase is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Individuals with hemizygous germline fumarate hydratase (FH) mutations are predisposed to renal cancer. These tumors predominantly exhibit functional inactivation of the remaining wild-type allele, implicating FH inactivation as a tumor-promoting event. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Fumarate Hydratase / FH protein (TMPY-02003) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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