Shopping Cart
- Remove All
- Your shopping cart is currently empty
Anti-LMAN2 Antibody (5W496) is a Mouse antibody targeting LMAN2. Anti-LMAN2 Antibody (5W496) can be used in WB,ELISA,ELISA(Det),IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $69 | 7-10 days | |
100 μL | $198 | 7-10 days |
Description | Anti-LMAN2 Antibody (5W496) is a Mouse antibody targeting LMAN2. Anti-LMAN2 Antibody (5W496) can be used in WB,ELISA,ELISA(Det),IP. |
Alias | VIP36, lectin, mannose binding 2, GP36B, C5orf8 |
Ig Type | Monoclonal Mouse IgG2a |
Clone | 5W496 |
Reactivity | Human |
Verified Activity | 1. Anti-LMAN2 mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:40 kDa. -Observed band size:40 kDa(We are unsure as to the identity of these extra bands.) 2. LMAN2-His was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -2 µL anti-LMAN2-His mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-LMAN2-His mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 40 kDa. -Observed band size: 40 kDa |
Application | WB,ELISA,ELISA(Det),IP |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate; ELISA(Det): 1:1000-1:10000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 / VIP36 (rh LMAN2 / VIP36; TMPY-02419; Q12907; Asp 45-Arg 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | LMAN2 (Lectin, Mannose Binding 2, also known as VIP36) is a Protein Coding gene. This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus, and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking, and quality control. The L-type lectin LMAN2 appears to be specifically required for the accumulation of GPRC5B in the Golgi complex and restriction of GPRC5B transport along the exosomal pathway. This may occur due to interference with the adaptor protein GGA1-mediated trans-Golgi network-to-endosome transport of GPRC5B. A Golgi-traversing pathway for the exosomal release of the cargo protein GPRC5B in which CD2AP facilitates the entry and LMAN2 impedes the exit of the flux, respectively. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human LMAN2 / VIP36 protein (TMPY-02419) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.