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Anti-LMAN2 Antibody (5W496)

😃Good
Catalog No. TMAY-02354
Alias VIP36, lectin, mannose binding 2, GP36B, C5orf8

Anti-LMAN2 Antibody (5W496) is a Mouse antibody targeting LMAN2. Anti-LMAN2 Antibody (5W496) can be used in WB,ELISA,ELISA(Det),IP.

Anti-LMAN2 Antibody (5W496)

Anti-LMAN2 Antibody (5W496)

😃Good
Catalog No. TMAY-02354Alias VIP36, lectin, mannose binding 2, GP36B, C5orf8
Anti-LMAN2 Antibody (5W496) is a Mouse antibody targeting LMAN2. Anti-LMAN2 Antibody (5W496) can be used in WB,ELISA,ELISA(Det),IP.
Pack SizePriceAvailabilityQuantity
20 μL$69 7-10 days
100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-LMAN2 Antibody (5W496) is a Mouse antibody targeting LMAN2. Anti-LMAN2 Antibody (5W496) can be used in WB,ELISA,ELISA(Det),IP.
AliasVIP36, lectin, mannose binding 2, GP36B, C5orf8
Ig Type
Monoclonal Mouse IgG2a
Clone
5W496
Reactivity
Human
Verified Activity
1. Anti-LMAN2 mouse monoclonal antibody at 1:500 dilution.

-Lane A: Jurkat Whole Cell Lysate.

-Lane B: Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:40 kDa.

-Observed band size:40 kDa(We are unsure as to the identity of these extra bands.)

2. LMAN2-His was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-2 µL anti-LMAN2-His mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-LMAN2-His mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 40 kDa.

-Observed band size: 40 kDa
Application
WB,ELISA,ELISA(Det),IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate; ELISA(Det): 1:1000-1:10000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 / VIP36 (rh LMAN2 / VIP36; TMPY-02419; Q12907; Asp 45-Arg 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundLMAN2 (Lectin, Mannose Binding 2, also known as VIP36) is a Protein Coding gene. This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus, and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking, and quality control. The L-type lectin LMAN2 appears to be specifically required for the accumulation of GPRC5B in the Golgi complex and restriction of GPRC5B transport along the exosomal pathway. This may occur due to interference with the adaptor protein GGA1-mediated trans-Golgi network-to-endosome transport of GPRC5B. A Golgi-traversing pathway for the exosomal release of the cargo protein GPRC5B in which CD2AP facilitates the entry and LMAN2 impedes the exit of the flux, respectively.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human LMAN2 / VIP36 protein (TMPY-02419)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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