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Anti-EIF4A1 Antibody (5Z864)
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Catalog No. TMAH-00382
Anti-EIF4A1 Antibody (5Z864) is an antibody targeting EIF4A1. Anti-EIF4A1 Antibody (5Z864) can be used in ELISA, WB, IF.
Anti-EIF4A1 Antibody (5Z864)
Anti-EIF4A1 Antibody (5Z864)
😃Good
Catalog No. TMAH-00382
Anti-EIF4A1 Antibody (5Z864) is an antibody targeting EIF4A1. Anti-EIF4A1 Antibody (5Z864) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Ig Type | Rabbit IgG |
| Clone | 5Z864 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate -All lanes: eIF4A1 Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 40, 47 kDa -Observed band size: 50 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-00382 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human eIF4A1 |
| Antigen Species | Human |
| Gene ID | 1973 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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