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Anti-Flag Tag Antibody-Biotin (3S989) (Synonyms: flagtag, DYKDDDDK-tag)

Catalog No. TMAS-00123B Copy Product Info
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Anti-Flag Tag Antibody-Biotin (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-Biotin (3S989) can be used in ELISA, WB.

Anti-Flag Tag Antibody-Biotin (3S989)

Copy Product Info
🥰Excellent
Catalog No. TMAS-00123B
Synonyms flagtag, DYKDDDDK-tag

Anti-Flag Tag Antibody-Biotin (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-Biotin (3S989) can be used in ELISA, WB.

Anti-Flag Tag Antibody-Biotin
(3S989)
Pack SizePriceUSA StockGlobal StockQuantity
40 μg$6097-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Flag Tag Antibody-Biotin (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-Biotin (3S989) can be used in ELISA, WB.
Synonymsflagtag, DYKDDDDK-tag
Ig Type
IgG
Clone
3S989
Specificity
DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins
Verified Activity
1. Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) binds with DYKDDDDK Tag.
Coating antigen: streptavidin, 5 µg/ml.
Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) dilution start from 1,000 ng/ml.
Secondary Antibody: Mouse Anti-Rabbit IgG Antibody [HRP], mAb.
EC50= 83.47 ng/ml.
2. Sensitivity and specificity of Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) to N-DYKDDDDK-tag Fusion Protein by Western blot.
Lane 1: N-terminal DYKDDDDK-tagged fusion protein (25 ng).
Lane 2: N-terminal DYKDDDDK-tagged fusion protein (10 ng).
Lane 3: N-terminal DYKDDDDK-tagged fusion protein (5 ng).
Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng).
Primary Antibody: Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) 1 μg/ml.
Secondary Antibody: Streptavidin-HRP 0.2 μg/ml.
3. Sensitivity and specificity of Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) to C-DYKDDDDK-tag Fusion Protein by Western blot.
Lane 1: C-terminal DYKDDDDK-tagged fusion protein (25 ng).
Lane 2: C-terminal DYKDDDDK-tagged fusion protein (10 ng).
Lane 3: C-terminal DYKDDDDK-tagged fusion protein (5 ng).
Lane 4: C-terminal DYKDDDDK-tagged fusion protein (1 ng).
Primary Antibody: Anti-Flag Tag Antibody-Biotin (3S989) (TMAS-00123B) 1 μg/ml.
Secondary Antibody: Streptavidin-HRP 0.2 μg/ml.
Application
Recommended Dose
ELISA: 0.2-0.5 μg/ml; WB: 1 μg/ml
Antibody Type
Monoclonal
Host SpeciesRabbit
PurificationProtein A affinity column
AppearanceLyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
FormulationLyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.
Research Background0
Related Conjugates and Formulations
Conjucates
Biotin
Others Formats
Unconjugated/HRP/FITC
Antibody Types Available
4
Antigen Details
Immunogen
KLH conjugated synthetic peptide: DYKDDDDK
Chemical Properties
Stability & Storage
Stability & StorageThe lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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