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IFN gamma Protein, Human, Recombinant (His & Avi)

IFN gamma Protein, Human, Recombinant (His & Avi)
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IFN gamma Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00047
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579.
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Pack SizePriceAvailabilityQuantity
100 μg$184In Stock
500 μg$7457-10 days
1 mg$1,2407-10 days
Bulk & Custom
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Biological Description

Biological Information
Immobilized Human IFN gamma, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 32.7 ng/mL determined by ELISA.
Description
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. IFN gamma Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 19.1 kDa and the accession number is P01579.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP01579
Synonyms
IFN gamma,interferon,Interferon-γ,γ,IFI,Interferon-gamma,IFG,IFN γ/IFNG,gamma,IFNγ,IFN γ
Construction
Gln24-Gly161
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight19.1 kDa (predicted). Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords