TIGIT Protein, Rat, Recombinant (hFc)

Name: TIGIT Protein, Rat, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-05744
Rating: 4.5 (1 reviews)

(Synonyms: T cell immunoreceptor with Ig and ITIM domains, RGD1563191) Copy Product Info

Synonyms: T cell immunoreceptor with Ig and ITIM domains, RGD1563191

Catalog No. TMPY-05744 Copy Product Info

TIGIT Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.02 kDa and the accession number is XP_008766987.1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days
200 µg	$1,090	7-10 days	7-10 days
500 µg	$2,260	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized Nectin-2 Protein, Human, Recombinant (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind TIGIT Protein, Rat, Recombinant (hFc Tag), the EC50 of TIGIT Protein, Rat, Recombinant (hFc Tag)is 60-300 ng/mL.
Description	TIGIT Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 40.02 kDa and the accession number is XP_008766987.1.
Species	Rat
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	XP_008766987.1
Construction	A DNA sequence encoding the rat TIGIT (XP_008766987.1) (Met1-Ala138) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Ala 17
Protein Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	T cell immunoreceptor with Ig and ITIM domains, RGD1563191
Research Background	TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

40.02 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.