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Anti-GAPDH Antibody (1K747)
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Catalog No. TMAH-00473
Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (1K747) is an antibody targeting GAPDH. Anti-GAPDH Antibody (1K747) can be used in ELISA, WB.
Anti-GAPDH Antibody (1K747)
Anti-GAPDH Antibody (1K747)
😃Good
Catalog No. TMAH-00473Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
Anti-GAPDH Antibody (1K747) is an antibody targeting GAPDH. Anti-GAPDH Antibody (1K747) can be used in ELISA, WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-GAPDH Antibody (1K747) is an antibody targeting GAPDH. Anti-GAPDH Antibody (1K747) can be used in ELISA, WB. |
| Alias | HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD |
| Ig Type | Rabbit IgG |
| Clone | 1K747 |
| Reactivity | Human, Mouse |
| Verified Activity | Western Blot -Positive WB detected in U87 whole cell lysate, Mouse brain tissue -All lanes GAPDH antibody at 0.31μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 36 KDa -Observed band size: 36 KDa |
| Application | ELISA, WB |
| Recommended Dose | WB:1:3000-1:10000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide |
| Antigen Species | Human |
| Gene ID | 2597 |
| Uniprot ID | |
| Biology Area | Neuroscience |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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