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Anti-PPM1G Antibody (6W278)
😃Good
Catalog No. TMAY-01795
Alias protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG
Anti-PPM1G Antibody (6W278) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (6W278) can be used in WB,ELISA,IP.
Anti-PPM1G Antibody (6W278)
Anti-PPM1G Antibody (6W278)
😃Good
Catalog No. TMAY-01795Alias protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG
Anti-PPM1G Antibody (6W278) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (6W278) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PPM1G Antibody (6W278) is a Rabbit antibody targeting PPM1G. Anti-PPM1G Antibody (6W278) can be used in WB,ELISA,IP. |
| Alias | protein phosphatase, Mg2+/Mn2+ dependent, 1G, PPP2CG, PP2Cγ, PP2CGAMMA, PP2CG |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6W278 |
| Reactivity | Human |
| Verified Activity | 1. Anti-PPM1G rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: 293T Whole Cell Lysate. -Lane C: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:59 kDa. -Observed band size:70 kDa. 2. PPM1G was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate. -Lane C:0.5 mg Jurkat Whole Cell Lysate. -2 µL anti-PPM1G rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PPM1G rabbit monoclonal antibody, at 1:50 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 59 kDa. -Observed band size: 72 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human PPM1G (rh PPM1G; TMPY-02197; O15355; Met317-Asp546). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein that belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human PPM1G protein (TMPY-02197) |
| Antigen Species | Human |
| Biology Area | Phosphatases and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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