Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IHC, IP

Reactivity: Human

Rabbit IgG

1Z917

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate
-All lanes: ARNT antibody at 1.83μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 87, 85, 86 KDa
-Observed band size: 87 KDa
2. IHC image of TMAH-00082 diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. Immunoprecipitating HIF-1 beta in Hela whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00082 in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00082 (3μg) + Hela whole cell lysate (500μg)
-Lane 3: Hela whole cell lysate (20μg)

ELISA, WB, IHC, IP

WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human ARNT

Human

405

Cardiovascular