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Anti-CD45 Antibody (3P27)
😃Good
Catalog No. TMAY-00244
Alias T200, protein tyrosine phosphatase, receptor type, C, LY5, L-CA, LCA, GP180, CD45R, CD45, B220
Anti-CD45 Antibody (3P27) is a Mouse antibody targeting CD45. Anti-CD45 Antibody (3P27) can be used in ELISA,FCM,ICC/IF.
Anti-CD45 Antibody (3P27)
Anti-CD45 Antibody (3P27)
😃Good
Catalog No. TMAY-00244Alias T200, protein tyrosine phosphatase, receptor type, C, LY5, L-CA, LCA, GP180, CD45R, CD45, B220
Anti-CD45 Antibody (3P27) is a Mouse antibody targeting CD45. Anti-CD45 Antibody (3P27) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $93 | 7-10 days | |
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, FCM, ICC/IF Reactivity: Human |
| Alias | T200, protein tyrosine phosphatase, receptor type, C, LY5, L-CA, LCA, GP180, CD45R, CD45, B220 |
| Ig Type | Mouse IgG1 |
| Clone | 3P27 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of Human CD45 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD45, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of Human CD45 in jurkat cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human CD45 monoclonal antibody (dilution ratio 1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to membrane. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD45 (rh CD45; TMPY-00711; NP_563578.2; Met1-Lys416). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases. |
| Conjucates | Unconjugated |
| Others Formats | APC/Biotin/PerCP/FITC/PE |
| Antibody Types Available | 6 |
| Immunogen | Recombinant Protein: Human CD45 protein (TMPY-00711) |
| Antigen Species | Human |
| Biology Area | Phosphatases and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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