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Anti-HIF-3 alpha Polyclonal Antibody
😃Good
Catalog No. TMAB-00860
Anti-HIF-3 alpha Polyclonal Antibody is a Rabbit antibody targeting HIF-3 alpha. Anti-HIF-3 alpha Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Anti-HIF-3 alpha Polyclonal Antibody
Anti-HIF-3 alpha Polyclonal Antibody
😃Good
Catalog No. TMAB-00860
Anti-HIF-3 alpha Polyclonal Antibody is a Rabbit antibody targeting HIF-3 alpha. Anti-HIF-3 alpha Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,ICC/IF,IF,ELISA Reactivity: Human,Mouse,Rat (predicted:Horse,Cow,Pig,Dog) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Horse,Cow,Pig,Dog) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HIF3 alpha) Polyclonal Antibody, Unconjugated (TMAB-00860) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HIF3 alpha) polyclonal Antibody, Unconjugated (TMAB-00860) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | IHC-P,IHC-Fr,ICC/IF,IF,ELISA |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide: human HIF3 alpha |
| Antigen Species | Human |
| Gene ID | 64344 |
| Uniprot ID | |
| Biology Area | HIF,HIF,HIF,HLH |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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