1. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide(NGD+) to cyclic GDPribose. The specific activity is > 2,500 pmoles/min/μg. 2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His at 2 μg/ml (100 μl/well) can bind Mouse Anti-CD38 Antibody , the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL. 3.Captured Daratumumab on anti-human IgG Fc via CM5 Chip can bind CD38 with an affinity constant of 5.429 nM as determined in a SPR assay(Routinely tested).

CD38 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.3 kDa and the accession number is P28907-1.

Human

HEK293 Cells

T10,CD38 molecule,ADPRC1,ADPRC 1

A DNA sequence encoding the extracellular domain of human CD38 (NP_001766.2) (Val 43-Ile 300) with a C-terminal polyhistidine tag was expressed. Predicted N terminal: Val 43

> 97 % as determined by SDS-PAGE.≥ 95 % as determined by SEC-HPLC.

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.