IFN gamma Protein, Mouse, Recombinant (hFc)

Name: IFN gamma Protein, Mouse, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-02322
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: interferon, γ, interferon, gamma, IFN-g, IFN γ, Ifg) Copy Product Info

Synonyms: interferon, γ, interferon, gamma, IFN-g, IFN γ, Ifg

Catalog No. TMPY-02322 Copy Product Info

IFN gamma Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.5 kDa and the accession number is P01580.

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	-	In Stock
10 µg	$74	-	In Stock
20 µg	$119	-	In Stock
50 µg	$229	-	In Stock
100 µg	$383	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 92 % as determined by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 2.5-15 ng/mL. 2. Measured by its ability to bind with recombinant mouse IFNGR1-His in a functional ELISA.
Description	IFN gamma Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.5 kDa and the accession number is P01580.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	P01580
Construction	A DNA sequence encoding the mouse IFNG (P01580) (Met 1-Cys 155) was fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: His 23
Protein Purity	> 92 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, 0.02% Tween 80, pH8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	interferon, γ, interferon, gamma, IFN-g, IFN γ, Ifg
Research Background	IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

42.5 kDa (predicted); 55 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.