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GDNF Protein, Human, Recombinant (HEK293)

Catalog No. TMPY-02792
Synonyms: HSCR3, ATF2, HFB1-GDNF, ATF1, glial cell derived neurotrophic factor

GDNF Protein, Human, Recombinant (HEK293) is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.1 kDa and the accession number is P39905-2.

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GDNF Protein, Human, Recombinant (HEK293)
Pack Size Availability Price/USD Quantity
10 μg In stock $ 106.00
20 μg 5 days $ 153.00
50 μg 5 days $ 242.00
100 μg 5 days $ 346.00
200 μg 5 days $ 613.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information 1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1 / GDNFRA Protein (His Tag) , the EC50 is 15-48 ng/mL.

Description GDNF Protein, Human, Recombinant (HEK293) is expressed in HEK293 mammalian cells. The predicted molecular weight is 15.1 kDa and the accession number is P39905-2.
Species Human
Expression System HEK293 Cells
Tag Tag Free
Accession Number P39905-2
Synonyms HSCR3, ATF2, HFB1-GDNF, ATF1, glial cell derived neurotrophic factor
Construction The mature form of Human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
Protein Purity > 97 % as determined by SDS-PAGE

Molecular Weight 15.1 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background Glial cell line-derived neurotrophic factor(GDNF) is an important member of the GDNF family of ligands(GFL). The GDNF family of ligands is comprised by four neurotrophic factors: glial cell line-derived neurotrophic factor (GDNF), neurturin (NRTN), artemin (ARTN), and persephin (PSPN). It has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. As the founding member, GDNF plays a key role in the promotion of the survival of dopaminergic neurons. GDNF is a highly conserved neurotrophic factor. The recombinant form of this protein also promotes the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption. It has been shown that GDNF results in two Parkinson's disease clinical trial and in a number of animal trials. It has been taken as a potent survival factor for central motoneurons.

References and Literature

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Keywords

GDNF Protein, Human, Recombinant (HEK293) HSCR3 HSCR 3 HSCR-3 ATF-1 ATF2 HFB1-GDNF ATF 2 ATF1 ATF-2 glial cell derived neurotrophic factor ATF 1 recombinant recombinant-proteins proteins protein

 

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