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Anti-DDX5 Antibody (4I744)
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Catalog No. TMAH-00337
Anti-DDX5 Antibody (4I744) is an antibody targeting DDX5. Anti-DDX5 Antibody (4I744) can be used in ELISA, WB, IHC, IF, FC.
Anti-DDX5 Antibody (4I744)
Anti-DDX5 Antibody (4I744)
😃Good
Catalog No. TMAH-00337
Anti-DDX5 Antibody (4I744) is an antibody targeting DDX5. Anti-DDX5 Antibody (4I744) can be used in ELISA, WB, IHC, IF, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-DDX5 Antibody (4I744) is an antibody targeting DDX5. Anti-DDX5 Antibody (4I744) can be used in ELISA, WB, IHC, IF, FC. |
| Ig Type | Rabbit IgG |
| Clone | 4I744 |
| Reactivity | Human, Mouse |
| Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, HT-29 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue -All lanes: DDX5 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 70, 61 kDa -Observed band size: 70 kDa 2. IHC image of TMAH-00337 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of HepG2 Cells with TMAH-00337 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). 4. Overlay histogram showing Hela cells stained with TMAH-00337 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, IHC, IF, FC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; FC:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Nucleus, nucleolus. Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human DDX5 |
| Antigen Species | Human |
| Gene ID | 1655 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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