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Anti-AARSD1 Antibody (3K421)
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Catalog No. TMAY-02713
Alias MGC2744, AlaXp, AARSD 1
Anti-AARSD1 Antibody (3K421) is a Rabbit antibody targeting AARSD1. Anti-AARSD1 Antibody (3K421) can be used in WB,ELISA,ICC/IF.
Anti-AARSD1 Antibody (3K421)
Anti-AARSD1 Antibody (3K421)
😃Good
Catalog No. TMAY-02713Alias MGC2744, AlaXp, AARSD 1
Anti-AARSD1 Antibody (3K421) is a Rabbit antibody targeting AARSD1. Anti-AARSD1 Antibody (3K421) can be used in WB,ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, ICC/IF Reactivity: Human |
| Alias | MGC2744, AlaXp, AARSD 1 |
| Ig Type | Rabbit IgG |
| Clone | 3K421 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AARSD1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
2. Anti-AARSD1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:45 kDa. -Observed band size:50 kDa |
| Application | WB,ELISA,ICC/IF |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human AARSD1 (Catalog #14280-H07E; Q9BTE6-2; Thr176-Glu525). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | AARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_25267.3, NM_113642.2, NM_1142653.1 and NM_1142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:1885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:188585. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human AARSD1 Protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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