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Anti-AARSD1 Antibody (3K421)

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Catalog No. TMAY-02713
Alias MGC2744, AlaXp, AARSD 1

Anti-AARSD1 Antibody (3K421) is a Rabbit antibody targeting AARSD1. Anti-AARSD1 Antibody (3K421) can be used in WB,ELISA,ICC/IF.

Anti-AARSD1 Antibody (3K421)

Anti-AARSD1 Antibody (3K421)

😃Good
Catalog No. TMAY-02713Alias MGC2744, AlaXp, AARSD 1
Anti-AARSD1 Antibody (3K421) is a Rabbit antibody targeting AARSD1. Anti-AARSD1 Antibody (3K421) can be used in WB,ELISA,ICC/IF.
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100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Anti-AARSD1 Antibody (3K421) is a Rabbit antibody targeting AARSD1. Anti-AARSD1 Antibody (3K421) can be used in WB,ELISA,ICC/IF.
AliasMGC2744, AlaXp, AARSD 1
Ig Type
Monoclonal Rabbit IgG
Clone
3K421
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AARSD1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Anti-AARSD1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: A549 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:45 kDa.

-Observed band size:50 kDa
Application
WB,ELISA,ICC/IF
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human AARSD1 (Catalog #14280-H07E; Q9BTE6-2; Thr176-Glu525).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundAARSD1 belongs to the class-II aminoacyl-tRNA synthetase family, Alax-L subfamily. AARSD1 binds 1 zinc ion per subunit functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala). Four transcript variants have been described for AARSD1: NM_25267.3, NM_113642.2, NM_1142653.1 and NM_1142654.1. It has been determined that the latter two variants represent a distinct upstream locus, which is now represented by GeneID:1885848 (PTGES3L), while the former two variants represent readthrough transcripts between PTGES3L and this locus (AARSD1). The readthrough locus (PTGES3L-AARSD1) is now represented by GeneID:188585.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human AARSD1 Protein
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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