TNFR1/CD120a/TNFRSF1A Protein, Mouse, Recombinant (hFc)

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Pack Size	Availability	Price/USD
100 μg	In stock	$ 386.00
200 μg	5 days	$ 682.00
500 μg	5 days	$ 1,440.00

Biological Information

1. Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-alpha at 2 μg/ml (100 μl/well) can bind Mouse TNFRSF1AhFc , the EC50 of Mouse TNFRSF1AhFc is 1.5-9.0 ng/mL. 2. Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 15-65 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNFα.

Description

Species	Mouse
Expression System	HEK293
Tag	hFc
Accession Number	P25118
Synonyms	TNFalpha-R1, TNFAR, TNF-R-I, TNF-R, TNFRp55, FPF, TNF-R55, TNFR60, TNFRI, tumor necrosis factor receptor superfamily member 1A, Tnfr-2, TNF-R1, TNF-αR1, Tnfr1, CD120a, TNF-alphaR1, p55, TNFα-R1, p55-R
Construction	A DNA sequence encoding the mouse TNFRSF1A (NP_ 035739.2) extracellular domain (Met 1-Ala 212) was fused with the Fc region of human IgG1 at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 47.4 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.