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R-Spondin 3/RSPO3 Protein, Human, Recombinant (His)

(Synonyms: THSD2, R-spondin 3, PWTSR, CRISTIN1) Copy Product Info

Synonyms: THSD2, R-spondin 3, PWTSR, CRISTIN1

Catalog No. TMPY-02115U Copy Product Info
R-Spondin 3/RSPO3 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q9BXY4-1.
R-Spondin 3/RSPO3 Protein, Human, Recombinant (His)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$887-10 days7-10 days
10 µg$1427-10 days7-10 days
20 µg$2337-10 days7-10 days
50 µg$4627-10 days7-10 days
100 µg$692-In Stock
200 µg$1,0807-10 days7-10 days
500 µg$1,8907-10 days7-10 days
1 mg$2,9507-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 95% as determined by Tris-Bis PAGE
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
1. Immobilized Human R-Spondin 3, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2 ng/ml determined by ELISA (QC Test).
2. Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test).
Description
R-Spondin 3/RSPO3 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q9BXY4-1.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberQ9BXY4-1
ConstructionGln22-Val146
Protein Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsTHSD2, R-spondin 3, PWTSR, CRISTIN1
Research Background
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Chemical Properties
Molecular Weight14.95 kDa (Predicted); 20-35 kDa (Due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: R-Spondin 3/RSPO3 Protein, Human, Recombinant (His) chemical structure | R-Spondin 3/RSPO3 Protein, Human, Recombinant (His) molecular weight