1. Immobilized Human R-Spondin 3, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2 ng/ml determined by ELISA (QC Test).
2. Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test).

R-Spondin 3/RSPO3 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q9BXY4-1.

Human

HEK293 Cells

THSD2,R-spondin 3,PWTSR,CRISTIN1

Gln22-Val146

> 95% as determined by Tris-Bis PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.