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Anti-JUNB Antibody (8U904) is an antibody targeting JUNB. Anti-JUNB Antibody (8U904) can be used in ELISA,IF,IHC.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $208 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
| Description | Anti-JUNB Antibody (8U904) is an antibody targeting JUNB. Anti-JUNB Antibody (8U904) can be used in ELISA,IF,IHC. |
| Synonyms | Transcription factor jun-B |
| Ig Type | Rabbit IgG |
| Clone | 8U904 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00658 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of MCF7 Cells with TMAH-00658 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | |
| Recommended Dose | IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human JunB |
| Antigen Species | Human |
| Gene Name | JUNB |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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