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Anti-HPX Antibody (2H197) is a Rabbit antibody targeting HPX. Anti-HPX Antibody (2H197) can be used in WB,ELISA,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $269 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Rat |
Alias | Hpxn, Hpx, Hemopexin |
Ig Type | Rabbit IgG |
Clone | 2H197 |
Reactivity | Rat |
Verified Activity | 1. Anti-HPX rabbit monoclonal antibody at 1:500 dilution. -Lane A: Rat kidney tissue Lysate. -Lane B: Rat spleen tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:52 kDa. -Observed band size:70 kDa. 2. ratHPX was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-ratHPX rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-ratHPX rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 52 kDa. -Observed band size:52 kDa |
Application | WB,ELISA,IP |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 5-10 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Rat Hemopexin/HPX (rR Hemopexin/HPX; TMPY-00160; P20059.3; Met1-Gln460). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Rat Hemopexin/HPX Protein (TMPY-00160) |
Antigen Species | Rat |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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