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Anti-SARS-CoV-2 Spike Antibody (3L24)
🥰Excellent
Catalog No. TMAY-02979
Anti-SARS-CoV-2 Spike Antibody (3L24) is a Mouse antibody targeting SARS-CoV-2 Spike. Anti-SARS-CoV-2 Spike Antibody (3L24) can be used in WB,ELISA,IHC-P.
Anti-SARS-CoV-2 Spike Antibody (3L24)
Anti-SARS-CoV-2 Spike Antibody (3L24)
🥰Excellent
Catalog No. TMAY-02979
Anti-SARS-CoV-2 Spike Antibody (3L24) is a Mouse antibody targeting SARS-CoV-2 Spike. Anti-SARS-CoV-2 Spike Antibody (3L24) can be used in WB,ELISA,IHC-P.
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Chemical Properties
| Description | Anti-SARS-CoV-2 Spike Antibody (3L24) is a Mouse antibody targeting SARS-CoV-2 Spike. Anti-SARS-CoV-2 Spike Antibody (3L24) can be used in WB,ELISA,IHC-P. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 3L24 |
| Verified Activity | 1. Anti-SARS-S1 mouse monoclonal antibody at 1:1000 dilution. -Lane A: SARS-CoV-2 (2019-nCoV) Spike S1 (30ng) -Lane B: SARS-CoV-2 (2019-nCoV) Spike S1 (5ng) -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. 2. Anti-SARS-CoV-2 (2019-nCoV) Spike mouse monoclonal antibody at 1:2000 dilution. -Lane A: SARS-COV-2 Spike (YP_009724390.1, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B: HEK293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) 3. Immunochemical analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-CoV-2 Spike Mouse Mab,then a HRP-conjugated second step antibody. (Validation Experiment) |
| Application | WB,ELISA,IHC-P |
| Recommended Dose | WB: 1:2000-1:5000; ELISA: 1:1000-1:2000; IHC-P: 1:500-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, Recombinant SARS-CoV-2 (2019-nCoV) Spike S1-mFc Protein (TMPY-05662; YP_009724390.1; Val16-Arg685). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Spike S1-mFc Protein (TMPY-05662) |
| Antigen Species | SARS-CoV-2 |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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