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HER2/ERBB2 Protein, Human, Recombinant
(Synonyms: TKR1, NGL, NEU, MLN19, MLN 19, HERV-W-ENV, HERVWENV, HERVW, HERV-7q, HERV7Q, HER-2/neu, HER-2, HER2, ERVWE1, erb-b2 receptor tyrosine kinase 2, ENVW, ENV, EGFR2, CD340) Copy Product InfoSynonyms: TKR1, NGL, NEU, MLN19, MLN 19, HERV-W-ENV, HERVWENV, HERVW, HERV-7q, HERV7Q, HER-2/neu, HER-2, HER2, ERVWE1, erb-b2 receptor tyrosine kinase 2, ENVW, ENV, EGFR2, CD340
Catalog No. TMPY-00167 Copy Product Info
HER2/ERBB2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 70 kDa and the accession number is P04626-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $60 | - | In Stock | |
| 10 μg | $95 | - | In Stock | |
| 20 μg | $153 | - | In Stock | |
| 50 μg | $293 | - | In Stock | |
| 100 μg | $503 | - | In Stock | |
| 200 μg | $889 | 7-10 days | 7-10 days | |
| 500 μg | $1,880 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:SDS-PAGE: 95.3%; SEC-HPLC: 99.5%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.3-1.5 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
2. Immobilized Human Her2/ERBB2 at 2 μg/ml (100 μl/well) can bind Herceptin, the EC50 of Herceptin is 7.0-30.0 ng/mL.   |
| Description | HER2/ERBB2 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 70 kDa and the accession number is P04626-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | P04626-1 |
| Construction | A DNA sequence encoding the human ERBB2 (NP_004439.2) (Met1-Thr652) was expressed with five amino acids (DDDDK) at the C-terminus. Predicted N terminal: Thr 23 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.   |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TKR1, NGL, NEU, MLN19, MLN 19, HERV-W-ENV, HERVWENV, HERVW, HERV-7q, HERV7Q, HER-2/neu, HER-2, HER2, ERVWE1, erb-b2 receptor tyrosine kinase 2, ENVW, ENV, EGFR2, CD340 |
| Research Background |
| Molecular Weight | 70 kDa (predicted); 100-110 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Related Tags: HER2/ERBB2 Protein, Human, Recombinant chemical structure | HER2/ERBB2 Protein, Human, Recombinant molecular weight
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