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Anti-SLC1A2 Antibody (9S258)

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Catalog No. TMAH-01098
Alias Solute carrier family 1 member 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/aspartate transporter II, GLT1, Excitatory amino acid transporter 2, EAAT2

Anti-SLC1A2 Antibody (9S258) is an antibody targeting SLC1A2. Anti-SLC1A2 Antibody (9S258) can be used in ELISA, WB, FC.

Anti-SLC1A2 Antibody (9S258)

Anti-SLC1A2 Antibody (9S258)

😃Good
Catalog No. TMAH-01098Alias Solute carrier family 1 member 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/aspartate transporter II, GLT1, Excitatory amino acid transporter 2, EAAT2
Anti-SLC1A2 Antibody (9S258) is an antibody targeting SLC1A2. Anti-SLC1A2 Antibody (9S258) can be used in ELISA, WB, FC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, FC

Reactivity: Human
AliasSolute carrier family 1 member 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/aspartate transporter II, GLT1, Excitatory amino acid transporter 2, EAAT2
Ig Type
Rabbit IgG
Clone
9S258
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: U251 whole cell lysate
-All lanes: EAAT2 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 62 kDa
-Observed band size: 62 kDa
2. Overlay Peak curve showing MCF-7 cells surface stained with TMAH-01098 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, WB, FC
Recommended Dose
WB:1:500-1:2000; FC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundSodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human SLC1A2
Antigen Species
Human
Gene ID
6506
Uniprot ID
Biology Area
Neuroscience, Metabolism, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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