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Anti-SLC1A2 Antibody (9S258) is an antibody targeting SLC1A2. Anti-SLC1A2 Antibody (9S258) can be used in ELISA, WB, FC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, FC Reactivity: Human |
Alias | Solute carrier family 1 member 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/aspartate transporter II, GLT1, Excitatory amino acid transporter 2, EAAT2 |
Ig Type | Rabbit IgG |
Clone | 9S258 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: U251 whole cell lysate -All lanes: EAAT2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62 kDa -Observed band size: 62 kDa 2. Overlay Peak curve showing MCF-7 cells surface stained with TMAH-01098 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, WB, FC |
Recommended Dose | WB:1:500-1:2000; FC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human SLC1A2 |
Antigen Species | Human |
Gene ID | 6506 |
Uniprot ID | |
Biology Area | Neuroscience, Metabolism, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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