Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated

Name: Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated
Brand: TargetMol
SKU: TMPY-06927
Rating: 4.5 (1 reviews)

(Synonyms: Harvey rat sarcoma viral oncogene homolog) Copy Product Info

Synonyms: Harvey rat sarcoma viral oncogene homolog

Catalog No. TMPY-06927 Copy Product Info

Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 59.58 kDa and the accession number is ACP41105.1.

Pack Size	Price	USA Stock	Global Stock
5 μg	$172	7-10 days	7-10 days
10 μg	$298	7-10 days	7-10 days
20 μg	$577	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Resource Download

User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Recombinant Influenza A H1N1 HA-specific B cell probe (Y108F,His & Avi Tag), Biotinylated (Cat#TMPY-06927) at 1 μg/mL (100 μl/well) on Streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-Influenza HA (Clone CR9114), the EC50 is 0.5-2 ng/mL (QC tested).
Description	Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 59.58 kDa and the accession number is ACP41105.1.
Species	H1N1
Expression System	Baculovirus Insect Cells
Tag	C-His-Avi
Accession Number	ACP41105.1
Construction	A DNA sequence encoding the Influenza A virus ((A/California/04/2009) (H1N1)) hemagglutinin (translated amino acids of ACP41105.1) (Y108F, Met1-Gly519), termed as HA-specific B cell probe, was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Asp 18
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonyms	Harvey rat sarcoma viral oncogene homolog
Research Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Chemical Properties

Molecular Weight

59.58 kDa (predicted); 66.9 kDa (reducing conditions)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated chemical structure | Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA-specific B cell probe (Y108F, His & Avi), Biotinylated molecular weight