Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF, FCM

Reactivity: Human, Rat

Rabbit IgG

3I476

Human, Rat

1. Western Blot
-Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue
-All lanes: MAS1L antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 43 kDa
-Observed band size: 36-50 kDa
2. Immunofluorescence staining of MCF-7 cell with TMAH-00738 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Immunofluorescence staining of Hela cell with TMAH-00738 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
4. Overlay Peak curve showing Hela cells stained with TMAH-00738 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions.

ELISA, WB, IF, FCM

WB:1:500-1:2000; IF:1:50-1:200; FCM:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human MAS1L

Human

116511

Neuroscience, Signal transduction