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Anti-C1QBP Antibody (6Q432)
😃Good
Catalog No. TMAY-02126
Alias P32, HABP1, gC1qBP, D11Wsu182e, complement component 1, q subcomponent binding protein, AA986492, AA407365
Anti-C1QBP Antibody (6Q432) is a Mouse antibody targeting C1QBP. Anti-C1QBP Antibody (6Q432) can be used in WB,ELISA,ICC/IF,IP.
Anti-C1QBP Antibody (6Q432)
Anti-C1QBP Antibody (6Q432)
😃Good
Catalog No. TMAY-02126Alias P32, HABP1, gC1qBP, D11Wsu182e, complement component 1, q subcomponent binding protein, AA986492, AA407365
Anti-C1QBP Antibody (6Q432) is a Mouse antibody targeting C1QBP. Anti-C1QBP Antibody (6Q432) can be used in WB,ELISA,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-C1QBP Antibody (6Q432) is a Mouse antibody targeting C1QBP. Anti-C1QBP Antibody (6Q432) can be used in WB,ELISA,ICC/IF,IP. |
| Alias | P32, HABP1, gC1qBP, D11Wsu182e, complement component 1, q subcomponent binding protein, AA986492, AA407365 |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 6Q432 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human C1QBP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human C1QBP monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. C1QBP was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-C1QBP mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-C1QBP mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 35 kDa. -Observed band size: 35 kDa. 3. Anti-C1QBP mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: 293T Whole Cell Lysate. -Lane C: Jurkat Whole Cell Lysate. -Lane D: NIH/3T3 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:31 kDa. -Observed band size:35 kDa. 4. Anti-C1QBP mouse monoclonal antibody at 1:500 dilution. -Lane A: C1QBP konckout Hela Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 10 μg per lane. -Secondary -Rabbit Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:31 kDa. -Observed band size:35 kDa(Validation Experiment) |
| Application | WB,ELISA,ICC/IF,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human C1QBP / HABP1 (rh C1QBP / HABP1; TMPY-01863; NP_001203.1; His 75-Gln 282). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Hyaluronan binding protein 1 (HABP1), also known as p32 or gC1qR, is a ubiquitously expressed multifunctional phospho-protein implicated in cell signalling. Hyaluronan-binding protein 1 (HABP1) /p32/gC1qR was characterized as a highly acidic and oligomeric protein, which binds to different ligands like hyaluronan, C1q, and mannosylated albumin. The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro. HABP1 overexpressing cells showed extensive vacuolation and reduced growth rate, which was corrected by frequent medium replenishment. Further investigation revealed that HABP1 overexpressing cells undergo apoptosis, and they failed to enter into the S-phase. The sperm surface HABP1 level can be correlated with the degree of sperm motility.Hyaluronan binding protein 1 (HABP1) was reported to be present on human sperm surface and its involvement in fertilization has already been elucidated: decreased HABP1 level may be associated with low motility of sperms, which in turn might cause infertility in the patient. HABP1 also is an endogenous substrate for MAP kinase and upon mitogenic stimulation it is translocated to the nucleus in a MAP kinase-dependent manner. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human C1QBP / HABP1 protein (TMPY-01863) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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