1. Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 μg/ml (100 μl/well) can bind human CD112 with a linear range of 6.4-800 ng/ml. 2. Immobilized PVRIG Protein, Cynomolgus, Rhesus, Recombinant (His Tag) at 2 μg/ml (100 μl/well) can bind Nectin-2 Protein, Human, Recombinant (hFc Tag), the EC50 of Nectin-2 Protein, Human, Recombinant (hFc Tag)is 0.2-1.2 ng/mL. 3.Loaded Human PVRIG Protein, mFc Tag on AMC Biosensor, can bind Human Nectin-2 protein, hFc Tag with an affinity constant of 8.784nM as determined in BLI assay (Routinely tested).

Nectin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 62.3 kDa and the accession number is Q92692-2.

Human

HEK293 Cells

Nectin-2,HVEB,poliovirus receptor-related 2 (herpesvirus entry mediator B),CD112,PRR2,PVRR2

The extracellular domain (Met 1-Leu 360) of Human CD112 (NP_002847.1) was fused with the Fc region of Human IgG1 at the C-terminus.

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.