KLKB1 Protein, Human, Recombinant (His)

Name: KLKB1 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPK-00766
Price: 56 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Plasma kallikrein, PKK, KLKB1, KLK3, Kininogenin, Fletcher factor) Copy Product Info

Synonyms: Plasma kallikrein, PKK, KLKB1, KLK3, Kininogenin, Fletcher factor

Catalog No. TMPK-00766 Copy Product Info

Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. KLKB1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 70.3 kDa and the accession number is P03952.

Pack Size	Price	USA Stock	Global Stock
5 µg	$56	-	In Stock
10 µg	$88	-	In Stock
20 µg	$143	7-10 days	7-10 days
50 µg	$278	7-10 days	7-10 days
100 µg	$465	-	In Stock
200 µg	$839	7-10 days	7-10 days
500 µg	$1,850	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:> 95%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human KLKB1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA.
Description	Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. KLKB1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 70.3 kDa and the accession number is P03952.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P03952
Construction	Gly20-Ala638
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM NaAc, 150 mM NaCl (pH 5.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	Plasma kallikrein, PKK, KLKB1, KLK3, Kininogenin, Fletcher factor
Research Background	Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.

Chemical Properties

Molecular Weight

70.3 kDa (predicted). Due to glycosylation, the protein migrates to 72-80 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.