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LAG-3 Protein, Mouse, Recombinant (His & Avi), Biotinylated
(Synonyms: Ly66, LAG-3, Lag3, CD223) Copy Product InfoSynonyms: Ly66, LAG-3, Lag3, CD223
Catalog No. TMPY-07222 Copy Product Info
LAG-3 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q61790.
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $208 | 7-10 days | 7-10 days | |
| 10 µg | $343 | 7-10 days | 7-10 days | |
| 20 µg | $588 | 7-10 days | 7-10 days | |
| 50 µg | $939 | 7-10 days | 7-10 days | |
| 100 µg | $1,600 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Recombinant Mouse LAG3 Protein (His & AVI Tag), Biotinylated at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Mouse FGL1 Protein (Fc Tag) with a linear range of 0.16-2.5 μg/mL |
| Description | LAG-3 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is Q61790. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q61790 |
| Construction | A DNA sequence encoding the Mouse LAG3 (NP_032505.1)(Met1-Leu442) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Protein Purity | ≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Please refer to the lot-specific COA. |
| Synonyms | Ly66, LAG-3, Lag3, CD223 |
| Research Background | LAG3 (Lymphocyte Activating 3) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| Molecular Weight | 48.5 kDa (predicted); 57.8 kDa (reducing contition) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: LAG-3 Protein, Mouse, Recombinant (His & Avi), Biotinylated chemical structure | LAG-3 Protein, Mouse, Recombinant (His & Avi), Biotinylated molecular weight
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