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Anti-CNN1 Antibody (5R138) is an antibody targeting CNN1. Anti-CNN1 Antibody (5R138) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human, Mouse |
Alias | smooth muscle, SMCC, Sm Calp, HEL S 14, Epididymis secretory protein Li 14, CNN 1, Calponins basic, Calponin-1, Calponin1, Calponin H1 smooth muscle, Calponin H1, Calponin 1 basic smooth muscle, Basic calponin |
Ig Type | Rabbit IgG |
Clone | 5R138 |
Reactivity | Human, Mouse |
Verified Activity | 1. Western Blot -Positive WB detected in: 293 whole cell lysate, Mouse lung tissue -All lanes: CNN1 antibody at 1.05μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34, 32 KDa -Observed band size: 34 KDa 2. IHC image of TMAH-00288 diluted at 1:105 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human CNN1 |
Antigen Species | Human |
Gene ID | 1264 |
Uniprot ID | |
Biology Area | Signal Transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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