B7-H3 Protein, Human, Recombinant (isoform 2, His)

B7-H3 Protein, Human, Recombinant (isoform 2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24.52 kDa and the accession number is Q5ZPR3-2.

Pack Size	Availability	Price/USD	Quantity
100 μg	5 days	$ 600.00
1 mg	5 days	$ 3,910.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 600.00

1 mg

5 days

$ 3,910.00

Description

B7-H3 Protein, Human, Recombinant (isoform 2, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 24.52 kDa and the accession number is Q5ZPR3-2.

Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q5ZPR3-2
Synonyms	CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, B7-H3
Construction	The Human CD276 (Q5ZPR3-2, isoform 2) (Met1-Thr243) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Molecular Weight	24.52 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy