Immobilized Human ITGAV&ITGB6, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human Latent TGF beta 1, His Tag with the EC50 of 0.49 μg/ml determined by ELISA.

Integrin alpha V beta 6 Protein, Human, Recombinant (Avi & His) is expressed in HEK293 Cells. The accession number is P06756-1(ITGAV)&P18564-1(ITGB6).

Human

HEK293 Cells

ITGAV & ITGB6,integrin, α5 (fibronectin receptor, α polypeptide),integrin, alpha 5 (fibronectin receptor, alpha polypeptide),Integrin α V β 6,Integrin alpha V β6

Phe31-Val992(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.