Antibody Type: Recombinant Monoclonal

Application: ELISA

Reactivity: Human

hIgG1

2V194

Human

1. The Binding Activity of Human AXL with Anti-AXL recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human AXL at 2 μg/mL can bind Anti-AXL recombinant antibody. The EC50 is 1.308-1.500 ng/mL.
2. Immunofluorescence staining of Hela cell with TMAH-00099 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
3. Immunofluorescence staining of PC-3 cell with TMAH-00099 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).
4. The purity of AXL was greater than 90% as determined by SEC-HPLC
5. 786-O cells were stained with Human IgG1&Igκ Isotype Control (green line) and anti-AXL antibody (TMAH-00099) (2µg/1*10^6 cells) (red line), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

ELISA

ELISA:1:5000-1:50000.

Monoclonal

Unconjugated

Recombinant Protein: Human AXL Protein

Human

558

Cancer, Cell biology, Tags & Cell Markers, Signal transduction