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Anti-Phospho-SMAD2 (Thr8) Polyclonal Antibody is a Rabbit antibody targeting Phospho-SMAD2 (Thr8). Anti-Phospho-SMAD2 (Thr8) Polyclonal Antibody can be used in WB,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $218 | 7-10 days | |
100 μL | $316 | 7-10 days |
Description | Anti-Phospho-SMAD2 (Thr8) Polyclonal Antibody is a Rabbit antibody targeting Phospho-SMAD2 (Thr8). Anti-Phospho-SMAD2 (Thr8) Polyclonal Antibody can be used in WB,IF,ELISA. |
Synonyms | SMAD2 (p-Thr8), SMAD2 (p-T8), SMAD2, p-SMAD2 (Thr8), p-SMAD2 (T8), Phospho-SMAD2 (T8), MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western Blot analysis of VEC cells using Phospho-Smad2/3 (T8) Polyclonal Antibody. 2. All lanes: Smad2/3 (Phospho-Thr8) Polyclonal Antibody at 1/500 dilution, Lane 1: 293T whole cell lysates, Lane 2: 293T treated with 20ng/ml TNFa for 30min whole cell lysates, Lane 3: 3T3 whole cell lysates, Lane 4: 3T3 treated with 20ng/ml TNFa for 30min whole cell lysates, Lane 5: Rat brain lysate, Lane 6: Mouse brain lysate, Lane 7: Rat liver lysate, Lane 8: Mouse liver lysate, Lane 9: Mouse kidney lysate Lysates at 20 μg per lane. Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 58-62 kDaExposure, time: 2 seconds. |
Application | |
Recommended Dose | WB: 1:500-2000; ELISA: 1:10000; ICC: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Appearance | Liquid |
Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | A synthesized phosphopeptide: human Smad2/3 around the phosphorylation site of T8 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Actual: 55-62 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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