1. Measured by its ability to bind biotinylated IL2 basic in a functional ELISA. 2. Measured by its ability to inhibit IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4 ng/mL of recombinant human IL-15. The ED50 for this effect is typically 2-12 μg/mL.

CD122/IL2RB Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 51 kDa and the accession number is P14784.

Human

HEK293 Cells

P70-75,interleukin 2 receptor, β,interleukin 2 receptor, beta,IL-15RB,IL15RB,CD122

A DNA sequence encoding the human IL2RB (NP_000869.1) (Met1-Asp239) was expressed with the Fc region of mouse IgG1 at the C-terminus. Predicted N terminal: Ala 27

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-2 receptor (IL-2R) also known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms concerning the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high-affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high-affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.