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Anti-DDOST Antibody (5B994)
😃Good
Catalog No. TMAY-02356
Alias WBP1, OST48, OST, OKSWcl45, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic), CDG1R, AGER1
Anti-DDOST Antibody (5B994) is a Mouse antibody targeting DDOST. Anti-DDOST Antibody (5B994) can be used in ELISA.
Anti-DDOST Antibody (5B994)
Anti-DDOST Antibody (5B994)
😃Good
Catalog No. TMAY-02356Alias WBP1, OST48, OST, OKSWcl45, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic), CDG1R, AGER1
Anti-DDOST Antibody (5B994) is a Mouse antibody targeting DDOST. Anti-DDOST Antibody (5B994) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-DDOST Antibody (5B994) is a Mouse antibody targeting DDOST. Anti-DDOST Antibody (5B994) can be used in ELISA. |
| Alias | WBP1, OST48, OST, OKSWcl45, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic), CDG1R, AGER1 |
| Ig Type | Monoclonal Mouse IgG2a |
| Clone | 5B994 |
| Reactivity | Human |
| Application | ELISA |
| Recommended Dose | ELISA: 1:1000-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human DDOST / OST48 (rh DDOST / OST48; TMPY-02050; P39656-1; Ser43-Pro427). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase) (DDOST), or 48-kDa subunit (OST48) is one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric complex composed of three type I transmembrane proteins of the endoplasmic reticulum: ribophorin I (RI), ribophorin II (RII), and OST48. OST48 is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum (ER). OST48 does not show significant amino acid sequence homology to either ribophorin I or II. It had been found that only the luminal domain of RI contains ER retention information. The dilysine motif in OST48 functions as an ER localization motif because OST48 in which the two lysine residues are replaced by serine (OST48ss) is no longer retained in the ER and is found instead also at the plasma membrane. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human DDOST / OST48 protein (TMPY-02050) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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