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Anti-HIF1A Antibody (2X244)
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Catalog No. TMAH-00543
Alias PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78
Anti-HIF1A Antibody (2X244) is an antibody targeting HIF1A. Anti-HIF1A Antibody (2X244) can be used in ELISA, IF, FC.
Anti-HIF1A Antibody (2X244)
Anti-HIF1A Antibody (2X244)
😃Good
Catalog No. TMAH-00543Alias PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78
Anti-HIF1A Antibody (2X244) is an antibody targeting HIF1A. Anti-HIF1A Antibody (2X244) can be used in ELISA, IF, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IF, FC Reactivity: Human |
| Alias | PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78 |
| Ig Type | Rabbit IgG |
| Clone | 2X244 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Hela cell with TMAH-00543 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing Hela cells stained with TMAH-00543 (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,001 events was performed. |
| Application | ELISA, IF, FC |
| Recommended Dose | IF:1:20-1:200; FC:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Nucleus speckle. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent proinflammatory state. In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons. Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human HIF1A Protein |
| Antigen Species | Human |
| Gene ID | 3091 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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