c-Kit/CD117 Protein, Mouse, Recombinant (His)

Name: c-Kit/CD117 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01802
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: W, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, Tr-kit, Ssm, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, Fdc, c-KIT, CD117, Bs) Copy Product Info

Synonyms: W, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, Tr-kit, Ssm, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, Fdc, c-KIT, CD117, Bs

Catalog No. TMPY-01802 Copy Product Info

Purity: 98.2%

c-Kit Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57 kDa and the accession number is P05532-1.

c-Kit/CD117 Protein, Mouse, Recombinant (His)

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	-	In Stock
10 µg	$75	-	In Stock
20 µg	$118	-	In Stock
50 µg	$229	-	In Stock
100 µg	$386	-	In Stock
200 µg	$682	7-10 days	7-10 days
500 µg	$1,430	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.2%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized mouse KIT at 2 μg/ml (100 μl/well) can bind biotinylated mouse KITL with a linear range of 1.28-6.4 ng/ml.
Description	c-Kit Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57 kDa and the accession number is P05532-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P05532-1
Construction	A DNA sequence encoding the mouse KIT isoform 1 (NP_001116205.1) extracellular domain (Met 1-Thr 523) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ser 25
Protein Purity	≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	W, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, Tr-kit, Ssm, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, Fdc, c-KIT, CD117, Bs
Research Background	C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

57 kDa (predicted); 70-80 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.