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Anti-HNRNPK Antibody (2G11)
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Catalog No. TMAH-00564
Alias TUNP, Transformation upregulated nuclear protein, Transformation up regulated nuclear protein, HNRNPK, hnRNP K, Heterogeneous nuclear ribonucleoprotein K, FLJ41122, dC stretch binding protein, CSBP
Anti-HNRNPK Antibody (2G11) is an antibody targeting HNRNPK. Anti-HNRNPK Antibody (2G11) can be used in ELISA, WB, IHC, IF, IP.
Anti-HNRNPK Antibody (2G11)
Anti-HNRNPK Antibody (2G11)
😃Good
Catalog No. TMAH-00564Alias TUNP, Transformation upregulated nuclear protein, Transformation up regulated nuclear protein, HNRNPK, hnRNP K, Heterogeneous nuclear ribonucleoprotein K, FLJ41122, dC stretch binding protein, CSBP
Anti-HNRNPK Antibody (2G11) is an antibody targeting HNRNPK. Anti-HNRNPK Antibody (2G11) can be used in ELISA, WB, IHC, IF, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IF, IP Reactivity: Human |
| Alias | TUNP, Transformation upregulated nuclear protein, Transformation up regulated nuclear protein, HNRNPK, hnRNP K, Heterogeneous nuclear ribonucleoprotein K, FLJ41122, dC stretch binding protein, CSBP |
| Ig Type | Rabbit IgG |
| Clone | 2G11 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate -All lanes: HNRNPK antibody at 1.3μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 51, 52, 49 KDa -Observed band size: 60 KDa 2. IHC image of TMAH-00564 diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. IHC image of TMAH-00564 diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 4. Immunofluorescence staining of A549 cells with TMAH-00564 at 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). 5. Immunoprecipitating HNRNPK in HepG2 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00564 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00564 (3μg) + HepG2 whole cell lysate (500μg) -Lane 3: HepG2 whole cell lysate (20μg) |
| Application | ELISA, WB, IHC, IF, IP |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus, nucleoplasm. Cell projection, podosome. Note=Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702). |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human HNRNPK |
| Antigen Species | Human |
| Gene ID | 3190 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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