Antibody Type: Recombinant Monoclonal

Application: ELISA, IHC, FC

Reactivity: Human

Rabbit IgG

8N3

Human

1. IHC image of TMAH-00377 diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.66% DAB.
2. Overlay Peak curve showing SH-SY5Y cells surface stained with TMAH-00377 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, IHC, FC

IHC:1:50-1:200; FC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human EGF

Human

1950

Cancer, Cell biology, Signal transduction