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Anti-ATF-5 Antibody (5U516)
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Catalog No. TMAH-00086
Anti-ATF-5 Antibody (5U516) is an antibody targeting ATF-5. Anti-ATF-5 Antibody (5U516) can be used in ELISA, WB, IHC.
Anti-ATF-5 Antibody (5U516)
Anti-ATF-5 Antibody (5U516)
😃Good
Catalog No. TMAH-00086
Anti-ATF-5 Antibody (5U516) is an antibody targeting ATF-5. Anti-ATF-5 Antibody (5U516) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-ATF-5 Antibody (5U516) is an antibody targeting ATF-5. Anti-ATF-5 Antibody (5U516) can be used in ELISA, WB, IHC. |
| Ig Type | Rabbit IgG |
| Clone | 5U516 |
| Reactivity | Human, Rat |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue -All lanes: ATF5 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 31 kDa -Observed band size: 35 kDa 2. IHC image of TMAH-00086 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1. Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human ATF5 |
| Antigen Species | Human |
| Gene ID | 22809 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Transcription factor-like protein ODA-10Transcription factor ATFxNRIF3-associated proteinNap1NAP 1Cyclic AMP-dependent transcription factor ATF-5cAMP-dependent transcription factor ATF-5BZIP protein ATF7BZIP protein ATF 7AtfxAtf5Atf 5Activating transcription factor 5-α/βActivating transcription factor 5-α/betaActivating transcription factor 5-α/bActivating transcription factor 5-alpha/βActivating transcription factor 5-alpha/betaActivating transcription factor 5-alpha/bActivating transcription factor 5-a/βActivating transcription factor 5-a/betaActivating transcription factor 5-a/b
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