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Anti-TSPAN8 Polyclonal Antibody
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Catalog No. TMAB-13874
Anti-TSPAN8 Polyclonal Antibody is a Rabbit antibody targeting TSPAN8. Anti-TSPAN8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Anti-TSPAN8 Polyclonal Antibody
Anti-TSPAN8 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-13874
Anti-TSPAN8 Polyclonal Antibody is a Rabbit antibody targeting TSPAN8. Anti-TSPAN8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-TSPAN8 Polyclonal Antibody is a Rabbit antibody targeting TSPAN8. Anti-TSPAN8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Sample: Lane 1: Mouse Pancreas tissue lysates Lane 2: Mouse Small intestine tissue lysates Lane 3: Human HeLa cell lysates Lane 4: Human HUVEC cell lysates Lane 5: Human SH-SY5Y cell lysates Lane 6: Human 293T cell lysates Primary: Anti-TSPAN8 (TMAB-13874) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 35 kDa 2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (TMAB-13874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (TMAB-13874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (TMAB-13874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TSPAN8) Polyclonal Antibody, Unconjugated (TMAB-13874) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; multi pass membrane protein. |
| Tissue Specificity | Gastric, colon, rectal, and pancreatic carcinomas. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human TSPAN8 |
| Antigen Species | Human |
| Gene Name | TSPAN8 |
| Gene ID | |
| Protein Name | Tetraspanin-8 |
| Uniprot ID |
| Molecular Weight | Theoretical: 26 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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