LAG-3 Protein, Human, Recombinant (His)

Name: LAG-3 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-04730
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: lymphocyte-activation gene 3, LAG-3, CD223) Copy Product Info

Synonyms: lymphocyte-activation gene 3, LAG-3, CD223

Catalog No. TMPY-04730 Copy Product Info

Purity: 98.5%

LAG-3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.6 kDa and the accession number is P18627-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$39	-	In Stock
10 µg	$59	7-10 days	7-10 days
20 µg	$93	-	In Stock
50 µg	$177	-	In Stock
100 µg	$311	7-10 days	7-10 days
200 µg	$549	7-10 days	7-10 days
500 µg	$1,160	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.5%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1. Immobilized LAG3 Protein, Human, Recombinant (ECD, His Tag) at 2 μg/mL (100 μL/well) can bind Anti-LAG3(BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL. 2.Captured Anti-LAG3 Mab (Human IgG4) on proA Chip can bind LAG3 with an affinity constant of 88.45 pM as determined in a SPR assay(Routinely tested) . 3.Loaded Anti-LAG-3 Mab(Human IgG4) on ProA Biosensor, can bind human LAG-3 protein, His Tag with an affinity constant of 0.617nM as determined in BLI assay (Routinely tested).
Description	LAG-3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.6 kDa and the accession number is P18627-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P18627-1
Construction	A DNA sequence encoding the human LAG3 (NP_002277.4) (Met1-Leu450) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 23
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	lymphocyte-activation gene 3, LAG-3, CD223
Research Background	LAG3 (Lymphocyte Activating 3) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

47.6 kDa (predicted); 55-60 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.