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Anti-NSDHL Antibody (1Q116)

😃Good
Catalog No. TMAH-00828
Alias Sterol-4-alpha-carboxylate 3-dehydrogenase, Protein H105e3, H105E3, EC 1.1.1.170, decarboxylating

Anti-NSDHL Antibody (1Q116) is an antibody targeting NSDHL. Anti-NSDHL Antibody (1Q116) can be used in ELISA, WB, IHC.

Anti-NSDHL Antibody (1Q116)

Anti-NSDHL Antibody (1Q116)

😃Good
Catalog No. TMAH-00828Alias Sterol-4-alpha-carboxylate 3-dehydrogenase, Protein H105e3, H105E3, EC 1.1.1.170, decarboxylating
Anti-NSDHL Antibody (1Q116) is an antibody targeting NSDHL. Anti-NSDHL Antibody (1Q116) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NSDHL Antibody (1Q116) is an antibody targeting NSDHL. Anti-NSDHL Antibody (1Q116) can be used in ELISA, WB, IHC.
AliasSterol-4-alpha-carboxylate 3-dehydrogenase, Protein H105e3, H105E3, EC 1.1.1.170, decarboxylating
Ig Type
Rabbit IgG
Clone
1Q116
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate
-All lanes: NSDHL antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 42 kDa
-Observed band size: 42 kDa
2. IHC image of TMAH-00828 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:2000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationEndoplasmic reticulum membrane; Single-pass membrane protein. Lipid droplet.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCatalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis. Plays also a role in the regulation of the endocytic trafficking of EGFR.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human NSDHL
Antigen Species
Human
Gene ID
50814
Uniprot ID
Biology Area
Cancer, Cardiovascular, Metabolism, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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