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ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-00664
ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 81.6 kDa and the accession number is O15123-1.
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50 μg $197In Stock
100 μg $3467-10 days
200 μg $6127-10 days
500 μg $1,3007-10 days
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Product Information

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml.
ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc)
Description
ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 81.6 kDa and the accession number is O15123-1.
Species
Human
Expression System
HEK293 Cells
TagC-hFc
Accession NumberO15123-1
Synonyms
Angiopoietin-2,angiopoietin 2,ANG2,AGPT2
Construction
A DNA sequence encoding the human Angiopoietin 2 (NP_001138.1) (Met 1-Phe 496) was fused with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Tyr 19
Protein Purity
> 90 % as determined by SDS-PAGE
ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc)
Molecular Weight81.6 kDa (predicted); 110-115 kDa (reducing condition, due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from sterile 20mM MOPS, 150mM NaCl, 0.2% CHAPS, pH7.0, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

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