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ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-00664
Synonyms: Angiopoietin-2, AGPT2, angiopoietin 2, ANG2

ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 81.6 kDa and the accession number is O15123-1.

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ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 197.00
100 μg 5 days $ 346.00
200 μg 5 days $ 612.00
500 μg 5 days $ 1,300.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Measured by its binding ability in a functional ELISA. Immobilized recombinant human Angiopoietin-2 at 10 μg/ml (100 μl/well) can bind Human Tie2 / Fc chimera with a range of 0.2-20 μg/ml.

Description ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 81.6 kDa and the accession number is O15123-1.
Species Human
Expression System HEK293 Cells
Tag C-hFc
Accession Number O15123-1
Synonyms Angiopoietin-2, AGPT2, angiopoietin 2, ANG2
Construction The Human Angiopoietin 2 (NP_001138.1) (Met 1-Phe 496) was fused with the Fc region of Human IgG1 at the C-terminus.
Protein Purity > 90 % as determined by SDS-PAGE

Molecular Weight 81.6 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

References and Literature

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Keywords

ANGPT2/Angiopoietin-2 Protein, Human, Recombinant (hFc) Angiopoietin-2 AGPT2 ANG-2 angiopoietin 2 AGPT 2 ANG 2 ANG2 AGPT-2 recombinant recombinant-proteins proteins protein

 

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