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Anti-ADAMTS5 Polyclonal Antibody
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Catalog No. TMAB-00057
Anti-ADAMTS5 Polyclonal Antibody is a Rabbit antibody targeting ADAMTS5. Anti-ADAMTS5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-ADAMTS5 Polyclonal Antibody
Anti-ADAMTS5 Polyclonal Antibody
😃Good
Catalog No. TMAB-00057
Anti-ADAMTS5 Polyclonal Antibody is a Rabbit antibody targeting ADAMTS5. Anti-ADAMTS5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,ELISA Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated (TMAB-00057) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Mouse Placenta tissue lysates Lane 2: Mouse Spleen tissue lysates Lane 3: Human U87MG cell lysates Lane 4: Human U251 cell lysates Lane 5: Human SH-SY5Y cell lysates Lane 6: Human HeLa cell lysates Primary: Anti-ADAMTS5 (TMAB-00057) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kDa Observed band size: 102 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and functions as an aggrecanase that cleaves aggrecan, a major proteoglycan of cartilage, and may mediate cartilage destruction in osteoarthritis. [provided by RefSeq, Feb 2016] |
| Immunogen | KLH conjugated synthetic peptide: human ADAMTS5 |
| Antigen Species | Human |
| Gene ID | 11096 |
| Uniprot ID | |
| Biology Area | Aggrecan,MMP,ADAM Protein Family,ADAM TS |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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