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Anti-CDX1 Antibody (3C463) is an antibody targeting CDX1. Anti-CDX1 Antibody (3C463) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
Alias | Homeobox protein CDX-1, CDX 1, Caudal-type homeobox protein 1 |
Ig Type | Rabbit IgG |
Clone | 3C463 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate -All lanes: CDX1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 29, 15 kDa -Observed band size: 25-30 kDa 2. Immunofluorescence staining of Hela cell with TMAH-00257 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 524-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Application | ELISA, WB, IF |
Recommended Dose | WB:1:500-1:2000; IF:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Plays a role in transcriptional regulation. Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in colorectal cancer (CRC) cells. Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human CDX1 |
Antigen Species | Human |
Gene ID | 1044 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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