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Anti-CDX1 Antibody (3C463)

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Catalog No. TMAH-00257
Alias Homeobox protein CDX-1, CDX 1, Caudal-type homeobox protein 1

Anti-CDX1 Antibody (3C463) is an antibody targeting CDX1. Anti-CDX1 Antibody (3C463) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.

Anti-CDX1 Antibody (3C463)

Anti-CDX1 Antibody (3C463)

😃Good
Catalog No. TMAH-00257Alias Homeobox protein CDX-1, CDX 1, Caudal-type homeobox protein 1
Anti-CDX1 Antibody (3C463) is an antibody targeting CDX1. Anti-CDX1 Antibody (3C463) can be used in ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF

Reactivity: Human
AliasHomeobox protein CDX-1, CDX 1, Caudal-type homeobox protein 1
Ig Type
Rabbit IgG
Clone
3C463
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: HepG2 whole cell lysate
-All lanes: CDX1 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 29, 15 kDa
-Observed band size: 25-30 kDa
2. Immunofluorescence staining of Hela cell with TMAH-00257 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 524-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Application
ELISA, WB, IF
Recommended Dose
WB:1:500-1:2000; IF:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundPlays a role in transcriptional regulation. Involved in activated KRAS-mediated transcriptional activation of PRKD1 in colorectal cancer (CRC) cells. Binds to the PRKD1 promoter in colorectal cancer (CRC) cells. Could play a role in the terminal differentiation of the intestine. Binds preferentially to methylated DNA.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human CDX1
Antigen Species
Human
Gene ID
1044
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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